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JoVE Journal Medicine
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
 
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Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)

Article DOI: 10.3791/3918-v • 11:54 min • April 29th, 2012
April 29th, 2012 •
1, 1, 1, 1, 1,2
1Department of Neurosurgery, The University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

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This video protocol demonstrates the application of the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) for the identification and separation of different sub-populations of cells in human glioblastoma based on frequency of cell division.

Tags

Slow-dividing Cells Identification Isolation Human Glioblastoma Carboxy Fluorescein Succinimidyl Ester (CFSE) Tumor Heterogeneity Therapeutic Strategies Phenotypic Identification Genetic Identification Functional Identification Tumor Subpopulations Disease Pathologies Clinically Relevant Targets Cell Division Frequency Brain Cancer Breast Cancer Skin Cancer Pancreas Cancer Leukemia Fluorescent Dye CFSE Tracking Cell Division Frequency Blood-borne Tumors Solid Tumors Glioblastoma
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