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Chapters
- 00:05Title
- 01:34Preparation of Mouse Embryonic Fibroblasts (MEF) Feeders
- 02:21Co-seeding hESCS onto MEFs
- 03:55Depleting MEFs from the Co-culture
- 04:50Results: hESCs Purified from Co-culture
- 06:40Conclusion
Trotz anhaltender Bemühungen, um den Übergang Kulturen Feeder-freien Bedingungen, bleiben die Ableitung und Kultur von humanen embryonalen Stammzellen (hES) weitgehend abhängig von Co-Kulturen mit embryonalen Feeder (MEF). Hier zeigen wir eine neuartige Methode zur schnellen Beseitigung Zubringer vom hESC Kulturen vor den Experimenten.
Tags
Fibroblast RemovalHigh Density Human Embryonic Stem Cell CulturesMouse Embryonic FibroblastsFeeder SystemHESC PurityModified Culture SystemsMitomycin C-treated Mouse Embryonic FibroblastsDifferentiation ExperimentsPurification TechniquesFACSMACSDrug Resistant VectorsPurer Population Of HESCsAspiration Of MEF Sheet
