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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

DOI:
10.3791/3973-v

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15:07 min

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August 05, 2012

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, , ,
1Department of Plastic and Hand Surgery,University of Freiburg Medical Centre

Chapters

  • 00:05Title
  • 02:04Anesthesia and Monitoring
  • 08:00Preparation of the Cremaster Muscle
  • 11:27Intravital Setup
  • 13:15Offline Video Playback Analysis
  • 13:59Conclusion

Summary

자동 번역

자동 번역

Digital intravital epifluorescence microscopy of postcapillary venules in the cremasteric microcirculation is a convenient method to gain insights into leukocyte-endothelial interaction in vivo in ischemia-reperfusion injury (IRI) of striated muscle tissue. We here provide a detailed protocol to safely perform the technique and discuss its applications and limitations.

Tags

Real-timeDigital ImagingLeukocyte-endothelial InteractionIschemia-reperfusion InjuryIRIRat Cremaster MusclePathological ConditionsCerebral StrokeMyocardial InfarctionIntestinal IschemiaTransplantCardiovascular SurgeryReactive Oxygen SpeciesComplement SystemMicrovascular PermeabilityLeukocyte ActivationAdhesion MoleculesChemoattractantsSelectinsCounter LigandsIntercellular Adhesion MoleculesVascular Cellular Adhesion MoleculesIntravital Microscopy

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