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Høst og Cryo-køling Krystaller af membranproteiner dyrket i Lipidiske mesofaser for Structure Bestemmelse ved makromolekylær krystallografi
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JoVE 신문 생물학
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Høst og Cryo-køling Krystaller af membranproteiner dyrket i Lipidiske mesofaser for Structure Bestemmelse ved makromolekylær krystallografi

DOI:
10.3791/4001-v

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18:45 min

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September 02, 2012

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, , , ,
1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology,Trinity College Dublin

Chapters

  • 00:00Title
  • 00:29Materials and Equipment
  • 02:12Identifying Wells With Crystals
  • 03:13Opening the Well
  • 04:04“Closeup”: Opening Wells with Cubic Phase – Method I
  • 06:38“Closeup”: Opening Wells with Cubic Phase – Method 2
  • 08:27Opening Well with Sponge Phase – Unsuccessfully
  • 10:25Opening Well with Sponge Phase – Successfully
  • 14:20Harvesting Crystals
  • 15:16Harvesting Crystals from the Cubic Phase
  • 16:12Harvesting Crystals from the Sponge Phase
  • 16:40Cryo-Cooling Crystals
  • 17:25Storage/Transport Dewars
  • 17:42Crystal Images
  • 18:03Concluding Remarks

Summary

자동 번역

자동 번역

Heri beskrives procedurer, der gennemføres i Caffrey Membrane strukturelle og funktionelle Biologi Group til at høste og cryo-cool membran proteinkrystaller dyrket i lipidiske kubiske og svamp faser til brug i strukturbestemmelse hjælp makromolekylære røntgenkrystallografi.

Tags

HarvestingCryo-coolingCrystalsMembrane ProteinsLipidic MesophasesStructure DeterminationMacromolecular CrystallographyIntegral Membrane ProteinsMacromolecular X-ray CrystallographyMX Diffraction Quality CrystalsG Protein-coupled Receptor FieldIn Meso MethodLipidic Cubic Phase MethodTechnical ChallengesViscous NatureSticky NatureMicro-crystalsManipulating CrystalsGlass Sandwich Plates

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