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Høsting og Cryo-kjøling Krystaller av membran proteiner Grown i Lipidic Mesophases for strukturbestemmelse av Macromolecular Crystallography
JoVE 신문
생물학
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JoVE 신문 생물학
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
DOI:

18:45 min

September 02, 2012

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Chapters

  • 00:00Title
  • 00:29Materials and Equipment
  • 02:12Identifying Wells With Crystals
  • 03:13Opening the Well
  • 04:04“Closeup”: Opening Wells with Cubic Phase – Method I
  • 06:38“Closeup”: Opening Wells with Cubic Phase – Method 2
  • 08:27Opening Well with Sponge Phase – Unsuccessfully
  • 10:25Opening Well with Sponge Phase – Successfully
  • 14:20Harvesting Crystals
  • 15:16Harvesting Crystals from the Cubic Phase
  • 16:12Harvesting Crystals from the Sponge Phase
  • 16:40Cryo-Cooling Crystals
  • 17:25Storage/Transport Dewars
  • 17:42Crystal Images
  • 18:03Concluding Remarks

Summary

자동 번역

Her er beskrevet rutiner er implementert i Caffrey Membran strukturelle og funksjonelle biologi, for å høste og Cryo-cool membran protein krystaller vokst i lipidic kubikk og svamp faser for bruk i strukturbestemmelse hjelp macromolecular røntgenkrystallografi.

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