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Generering af mus afledt fra induceret pluripotente stamceller
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JoVE 신문 생물학
Generation of Mice Derived from Induced Pluripotent Stem Cells

Generering af mus afledt fra induceret pluripotente stamceller

DOI:
10.3791/4003-v

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11:56 min

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November 29, 2012

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, , , , , ,
1Dorris Neuroscience Center & Department of Cell Biology,The Scripps Research Institute , 2Mouse Genetics Core Facility,The Scripps Research Institute

Chapters

  • 00:05Title
  • 02:12Preparation of Lentivirus
  • 03:24Derivation of iPSC Lines
  • 04:37Generation of Tetraploid Blastocysts
  • 07:20Preparation of iPSCs for Blastocyst Injection
  • 09:20Results: Reprogramming MEFs to iPSCs
  • 10:55Conclusion

Summary

자동 번역

자동 번역

Generering induceret pluripotente stamceller (IPSC) linjer producerer linjer af forskellig udviklingspotentiale, selv når de passerer standardtests for pluripotency. Her beskriver vi en protokol til at producere mus der udelukkende kommer fra iPSCs, der definerer de IPSC linjer som besidder fuld pluripotency<sup> 1</sup>.

Tags

Induced Pluripotent Stem CellsIPSCsBasic ResearchRegenerative TherapiesSomatic CellsGerm LayersEmbryoid BodiesTeratomasGene ExpressionChimeric MicePluripotency MarkersDonor Cell SourcesSelection MethodsTetraploid Embryo ComplementationFull PluripotencyElectrofusionBlastocyst Stage

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