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Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
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Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

DOI:
10.3791/4016-v

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13:56 min

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November 28, 2012

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, ,
1St. Erik’s Eye Hospital,Karolinska Institutet, 2Gullstrand lab, Section for Ophthalmology, Department of Neuroscience,Uppsala University

Chapters

  • 00:05Title
  • 02:00Exposure to Ultraviolet Radiation
  • 03:26Dissection
  • 04:31Quantitative RT-PCR
  • 08:56Immunohistochemical Staining
  • 10:06Sectioning and Fluorescence Immunohistochemistry
  • 11:41Representative qPCR and Imaging of UVR Exposed vs Non-exposed Lens
  • 13:30Conclusion

Summary

자동 번역

자동 번역

Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.

Tags

CataractBlindnessWorld Health OrganizationClouding Of The LensMulti-factorial DiseaseDiabetesSmokingUltraviolet Radiation (UVR)AlcoholIonizing RadiationSteroidsHypertensionAnimal ModelSurgeryPrevention StrategiesApoptosisLensPharmaceuticalsRT-PCRImmunohistochemistry

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