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Preparing T Cell Growth Factor from Rat Splenocytes

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Preparing T Cell Growth Factor from Rat Splenocytes

Preparing T Cell Growth Factor from Rat Splenocytes

DOI:

•

18:27 min

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October 31, 2007

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Christine Beeton, K. George Chandy

¹Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

00:00Title
00:06Introduction
01:13TCGF Secretion
03:22Harvesting Spleens from Lewis Rats
05:15Preparing Single Cell Suspensions from Rat Spleens
10:23Lysis of Red Blood Cells
12:40Preparing Splenocyte Culture
13:34Elimination of the Conconavalin A
17:53Conclusion

Summary

자동 번역

We describe the preparation of T cell growth factor used for the in vitro expansion of antigen-specific rat T lymphocyte lines.

Tags

T Cell Growth Factor Rat Splenocytes Antigen-specific T Cell Lines Interleukin 2 Th1 Polarization TCGF Preparation Cytokines Large-scale Production Rat Splenocyte Culture Supernatants Concanavalin A Mitogen Cytokine Production Sterile Filtration Storage At -20°C

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