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Chapters
- 00:05Title
- 02:02Induction of Fusion by PEG
- 05:04Immunofluorescence to Determine Fusion Efficiency
- 07:21Co-culture and Transfection of a Fusogenic PFV Glycoprotein
- 08:24Infection Assay
- 09:33Visualization of Cell Fusion
- 10:56Results: Using Heterokaryon Formation to Assess the Influence of Dominant Restrictions on the HCV Life Cycle
- 16:23Conclusion
Tags
Heterokaryon FormationHCV Restriction FactorsHCV ReplicationHost-rangeChimpanzeesHCV RNA ReplicationNon-hepatic Cell LinesMurine Cell LinesSelection ProceduresHCV Replicon ConstructsAntibiotic-selectable MarkersIn Vitro ResearchHepatoma Cell LinesViral Life CycleSpecies TropismImmunocompetent Small Animal ModelGenetic IncompatibilityHost Dependency FactorsRestriction FactorsConditional Trans-complementation MethodsSomatic Cell FusionViral Replication CycleHeterokaryonsTrans-complementationInfectious Viral ProgenySubgenomic HCV Rep
