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Dos Métodos de Formación heterocarión para descubrir los factores de restricción del VHC
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Chapters
- 00:05Title
- 02:02Induction of Fusion by PEG
- 05:04Immunofluorescence to Determine Fusion Efficiency
- 07:21Co-culture and Transfection of a Fusogenic PFV Glycoprotein
- 08:24Infection Assay
- 09:33Visualization of Cell Fusion
- 10:56Results: Using Heterokaryon Formation to Assess the Influence of Dominant Restrictions on the HCV Life Cycle
- 16:23Conclusion
Se describen dos métodos para la condicional<em> Trans</em>-La complementación de la hepatitis C (VHC) el montaje y la realización del ciclo de vida viral, que se basan en la formación de heterocarión. Estas técnicas son adecuadas para la detección de líneas celulares que expresan los factores dominantes de restricción, lo que impide la producción de progenie infecciosa del VHC.
Tags
Heterokaryon FormationHCV Restriction FactorsHCV ReplicationHost-rangeChimpanzeesHCV RNA ReplicationNon-hepatic Cell LinesMurine Cell LinesSelection ProceduresHCV Replicon ConstructsAntibiotic-selectable MarkersIn Vitro ResearchHepatoma Cell LinesViral Life CycleSpecies TropismImmunocompetent Small Animal ModelGenetic IncompatibilityHost Dependency FactorsRestriction FactorsConditional Trans-complementation MethodsSomatic Cell FusionViral Replication CycleHeterokaryonsTrans-complementationInfectious Viral ProgenySubgenomic HCV Rep
