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Chapters
- 00:05Title
- 01:32Initial Characterization and Considerations
- 03:04Spotting Cells onto Solid Agar Media
- 05:36Monitoring Colony Morphology by Microscopy
- 08:14Results: Analysis of Biofilm Development
- 10:49Conclusion
Vi tilvejebringe en enkel, semikvantitativ metode til at undersøge biofilmdannelse<em> In vitro</em>. Denne fremgangsmåde drager fordel af den Zeiss STEMI 2000-C dissektionsmikroskop (med kameraet fastgørelse) for at overvåge både timing og mønstret af biofilmdannelse, som vurderet ved udviklingen af rynkede kolonier.
Tags
Semi-quantitative ApproachAssess Biofilm FormationWrinkled Colony DevelopmentBacteriaExtracellular MatrixPhysiologyResistance To AntibioticsEnvironmental StressesHost-microbe InteractionsPlanktonic CellsComplex CommunitiesLaboratory StudyBiofilm PhenotypesWrinkled Or Rugose Bacterial ColoniesSolid Agar MediaIndicator Of Biofilm FormationGram-positive BacteriaGram-negative BacteriaBacillus SubtilisVibrio CholeraeVibrio ParahaemolyticusPseudomonas AeruginosaVibrio Fischeri
