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Chapters
- 00:01Title
- 00:17Introduction
- 01:07Hand-Picking the Islets
- 03:18Transferring Islets into P150 Tubing
- 05:17Preparing the Mouse for Islet Transplant
- 05:59Exposing the Kidney
- 07:16Transplantation of Islets
- 10:01Conclusion
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.
Tags
TransplantationPancreatic IsletsKidney CapsuleDiabetic MiceProtocolCellsDelivery TubingPE50ConcentrationPelletsSpeedEaseStress ReductionLoadingMicrocentrifuge TubePipettemanPipette TipPE50 Tubing AttachmentSilicone Adapter TubingSettlingDialingKink15 ML ConicalCentrifugingAnesthesiaShavingCleaningIncisionKidney Exposure
