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Chapters
- 00:05Title
- 01:18Acute Slices Preparation
- 04:25Time-lapse Imaging of Neuroblast Migration
- 05:50Analyzing Neuroblast Migration
- 08:46Results: Time-lapse Imaging of Neuroblast Migration in Acute Slice
- 09:23Conclusion
We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.
