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Chapters
- 00:05Title
- 01:12Microtomy for Unstained Sections
- 02:50H&E Staining
- 03:45Immunohistochemistry (IHC) Procedure
- 05:19Dehydration
- 06:16Slide Labeling and Scanning
- 07:05Results: Staining of Pancreatic Sections
- 07:32Conclusion
This video demonstrates procedures for characterization of human pancreatic islets using hematoxylin and eosin (H&E) and immunohistochemistry (IHC). Pancreatic sections from head, body, and tail regions are stained by both H&E and IHC to determine islet endocrine composition (insulin, glucagon, and pancreatic polypeptide), cell replication (Ki67), and inflammatory infiltrates (H&E, CD3). The uncinate region is localized using IHC for pancreatic polypeptide.
Tags
Staining ProtocolsHuman Pancreatic IsletsIslet AreaEndocrine Cell CompositionProcessing And Staining ProcedureHistopathologyImmunolocalization ExaminationsStandardized ProceduresTransverse SectionsPancreatic HeadPancreas TailSerial SectioningHistopathological CharacterizationIslet Endocrine CellsReplicationT-cell InfiltratesExocrine CompartmentDuctular Components
