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Chapters
- 00:05Title
- 01:51Preparation of Algal Material
- 04:05Electroporation
- 06:54Inclusion of Cells on Plates in Semi-solid Medium
- 08:46Selection of Transformed Colonies
- 09:41Results: Appearance of Colonies on Transformation Plates
- 10:05Conclusion
This article describes genetic transformation of the unicellular marine alga Ostreococcus tauri by electroporation. This eukaryotic organism is an effective model platform for higher plants, possesing greatly reduced genomic and cellular complexity and being readily amenable to both cell culture and chemical biology.
Tags
Genomic TransformationPicoeukaryote Ostreococcus TauriPlant SciencesCellular ComplexityWhole Organism ComplexityGenomic RedundancyModel OrganismGenome SizeCellular ComplexityOrganellesGenesUnicellularityEasy CultureChemical Biology ApproachesCircadian TimekeepingMammalian BiologyGreen LineageRapid ExperimentationTestable HypothesesGenome ModificationGenetic Transformation
