JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:22Ex ovo Culture
- 02:51Preparation for Ex ovo Electroporation
- 03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
- 04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
- 05:50Conclusion
A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.
