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Chapters
- 00:05Title
- 01:26Colitis-associated Cancer Induction
- 02:28Clinical Assessment of Colitis and Tumor Progression
- 02:59Murine Endoscopy(Optional)
- 04:24Mouse Sacrifice and Colon Harvesting
- 06:55Preparing the Colon for Macroscopic Analysis
- 07:55Preparing the Colon for Histological Assessment
- 09:21Representative Results
We demonstrate a protocol in which administration of the genotoxic agent azoxymethane (AOM) followed by three cycles of the pro-inflammatory agent dextran sulfate sodium (DSS) rapidly and consistently generates colon tumors in mice with morphologic and molecular similarities to those seen in human colitis-associated cancer.
