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高通量的亲和标签的重组蛋白的纯化
JoVE 신문
생물학
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JoVE 신문 생물학
High-throughput Purification of Affinity-tagged Recombinant Proteins
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高通量的亲和标签的重组蛋白的纯化

DOI:
10.3791/4110-v

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07:44 min

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August 26, 2012

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,
1Department of Life Sciences,Imperial College London

Chapters

  • 00:05Title
  • 01:27High-throughput Growth of Bacterial Cultures
  • 02:38Robotic Purification of Recombinant Proteins
  • 04:06Protein Purification
  • 06:32Representative Protein Purification Results
  • 07:08Conclusion

Summary

자동 번역

자동 번역

我们描述了一种方法，亲和标签的重组蛋白的纯化，使用的液体处理机器人。此方法通常是适用于小规模纯化的可溶性的带有His标记的蛋白质在高吞吐量的格式。

Tags

High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery

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