JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:54Generation of shRNA Constructs
- 04:33Lentivirus Transfection
- 06:35Infection and Characterization of Infected Cells
- 07:47Xenograft Mouse Model Study
- 10:39YY1 Knockdown Experiment
- 12:42Conclusion
RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in vivo.
