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Eine quantitative Auswertung der Zellmigration durch die Phagokinetic Spur Motilität Assay
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1Department of Microbiology and Immunology,Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology,Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology,SUNY Upstate Medical University, 4Feist-Weiller Cancer Center,Louisiana State University Health Sciences Center
Chapters
- 00:05Title
- 02:20Preparation of Gelatin-coated Coverslips
- 04:00Preparation of Colloidal Gold-coated Coverslips
- 07:41Analysis of Cellular Motility
- 09:40Results: Cell Motility Paths in Colloidal Gold Particles
- 10:33Conclusion
Die Motilität phagokinetic Spur Assay ist ein Verfahren verwendet, um die Bewegung von Zellen beurteilen. Insbesondere misst der Test Chemokinese (random Zellmotilität) über die Zeit in einer quantitativen Weise. Der Assay nutzt die Fähigkeit von Zellen, um eine messbare Spur ihrer Bewegung auf kolloidalem Gold beschichteten Deckgläsern erstellen.
Tags
Cell MigrationPhagokinetic Track Motility AssayQuantitative EvaluationCellular MotilityUnicellular OrganismsMulticellular OrganismsImmune SurveillanceWound HealingNeurological DiseasesCardiovascular DiseasesImmunological DiseasesTumor FormationActin PolymerizationReceptor RecyclingCellular ExtensionsLamellipodiaCell MotilityMicroscopyQuantitative MeasurementResource-consuming Process
