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Chapters
- 00:05Title
- 02:38Dissection
- 04:51Tissue Analysis: Total Protein and Final Normalization of Tyrosine Hydroxylase and Dopamine Active Transporter Results
- 08:12Results: Tyrosine Hydroxylase Phosphorylation in Rat Substantia Nigra and Ventral Tegmental Samples
- 09:00Conclusion
Dopamine is duidelijk geregeld in de middenhersenen kernen, die de cellichamen en dendrieten van de dopamine neuronen bevatten. Hier beschrijven we een dissectie en sample-handling aanpak om de resultaten te maximaliseren, en dus conclusies en inzichten, op dopamine regelgeving in de middenhersenen kernen van de substantia nigra (SN) en ventrale tegmentale gebied (VTA) bij knaagdieren.
Tags
Dopamine RegulationNeurotransmitterCNSLocomotor ActivityReward-related BehaviorMolecular DeficienciesNigrostriatal PathwayMesoaccumbens PathwayStriatumNucleus AccumbensTyrosine Hydroxylase ProteinTH PhosphorylationDopamine Transporter ProteinVesicular Monoamine Transporter 2 ProteinPost-translational ModificationsMidbrain
