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Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

DOI:
10.3791/4203-v

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07:50 min

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July 16, 2012

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, , , , ,
1Institute for Toxicology and Genetics,Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics,Karlsruhe Institute of Technology, Karlsruhe, Germany

Chapters

  • 00:05Title
  • 01:12Animal Handling and Media Preparation
  • 02:15Drug Treatment
  • 03:00Image Acquisition and Processing
  • 04:02Data Processing
  • 06:32Representative Inflammatory Assay Results
  • 07:23Conclusion

Summary

자동 번역

자동 번역

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.

Tags

Drug DiscoveryAutomated High-content Inflammation AssayZebrafishSmall Molecule ScreensImmune-modulatory CompoundsHigh-throughput ScreeningChemically Induced Inflammation AssayCopper Sulfate TreatmentGranulocyte Recruitment

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