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Chapters
- 00:05Title
- 01:52Solution Preparation and Brain Dissection
- 03:21Coronal Hypothalamic Sections Slicing
- 04:30Preparation of the Ca2+ Indicator Dye Loading Solution
- 06:35Microscopy and Analysis
- 07:59Results: Ca2+ Signals in TGFP Neurons of Mouse Hypothalamic Brain Slices
- 08:52Conclusion
Tags
ImagingCalcium ResponsesGFP-tagged NeuronsHypothalamic Mouse Brain SlicesEncoding Of InformationMolecular StepsNeurohormone Signal Transduction CascadesIntracellular Ca2+Synaptic SitesGreen Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ ResponsesNon-green Fluorescent Ca2+ Indicator DyeConfocal MicroscopyRatiometric Ca2+ Indicator Fura-2Ultraviolet (UV) Light
