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Imaging Calcium Antworten in GFP-markierten Neuronen des Hypothalamus Maus Hirnschnitten
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JoVE 신문 신경과학
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
DOI:

09:14 min

August 24, 2012

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Chapters

  • 00:05Title
  • 01:52Solution Preparation and Brain Dissection
  • 03:21Coronal Hypothalamic Sections Slicing
  • 04:30Preparation of the Ca2+ Indicator Dye Loading Solution
  • 06:35Microscopy and Analysis
  • 07:59Results: Ca2+ Signals in TGFP Neurons of Mouse Hypothalamic Brain Slices
  • 08:52Conclusion

Summary

자동 번역

In diesem Protokoll, aktualisieren wir die jüngsten Fortschritte in der Bildgebung Ca<sup> 2 +</sup> Signale GFP-markierten Neuronen im Gehirn Gewebeschnitte mit einem rot fluoreszierenden Ca<sup> 2 +</sup> Indikatorfarbstoff.

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