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๊ณผ๋ฆฝ์— ๋”ฐ๋ผ ๋‹ฌ๋ผ Autoantibody ์œ ๋ฐœ ํ”ผ๋ถ€ ์ –์–ด
JoVE ์‹ ๋ฌธ
๋ฉด์—ญํ•™ ๋ฐ ๊ฐ์—ผ๋ณ‘ํ•™
Author Produced
JoVE ๋น„๋””์˜ค๋ฅผ ํ™œ์šฉํ•˜์‹œ๋ ค๋ฉด ๋„์„œ๊ด€์„ ํ†ตํ•œ ๊ธฐ๊ด€ ๊ตฌ๋…์ด ํ•„์š”ํ•ฉ๋‹ˆ๋‹ค.  ์ „์ฒด ๋น„๋””์˜ค๋ฅผ ๋ณด์‹œ๋ ค๋ฉด ๋กœ๊ทธ์ธํ•˜๊ฑฐ๋‚˜ ๋ฌด๋ฃŒ ํŠธ๋ผ์ด์–ผ์„ ์‹œ์ž‘ํ•˜์„ธ์š”.
JoVE ์‹ ๋ฌธ ๋ฉด์—ญํ•™ ๋ฐ ๊ฐ์—ผ๋ณ‘ํ•™
Granulocyte-dependent Autoantibody-induced Skin Blistering
Please note that all translations are automatically generated. Click here for the English version.

๊ณผ๋ฆฝ์— ๋”ฐ๋ผ ๋‹ฌ๋ผ Autoantibody ์œ ๋ฐœ ํ”ผ๋ถ€ ์ –์–ด

DOI:
10.3791/4250-v

โ€ข

12:23 min

โ€ข

October 12, 2012

โ€ข

, ,
1Department of Dermatology,University of Freiburg , 2Kepler High School Freiburg, 3Centre for Biological Signalling Studies (BIOSS),University of Freiburg

Chapters

  • 00:00Title
  • 01:28Overall Scheme of the Experiment
  • 02:36Preparation of Pathogenic Antibody
  • 04:06Injection of Collagen VII-specific IgG into Mice
  • 06:47Clinical Evaluation of Disease Severity
  • 09:13Analysis of Tissue-bound and Circulation Collagen-specific Antibodies
  • 10:19Representative Results
  • 11:41Conclusions

Summary

์ž๋™ ๋ฒˆ์—ญ

์ž๋™ ๋ฒˆ์—ญ

์ฝœ๋ผ๊ฒ VII 200 ์•„๋ฏธ๋…ธ์‚ฐ (AA 757-967)์˜ ์ŠคํŠธ๋ ˆ์น˜์— ๋Œ€ํ•œ IgG ํ•ญ์ฒด๋ฅผ ์ •ํ™”, ์šฐ๋ฆฌ์˜ ํ˜„์žฌ ์ž‘์—…์— ์„ค๋ช… ๋œ ๋™๋ฌผ ๋ชจ๋ธ์—์„œ ์ –์–ด ํ‘œํ˜„ํ˜•๋ฟ๋งŒ ์•„๋‹ˆ๋ผ ์ธ๊ฐ„์„ ํŠน์ง• ์กฐ์ง์„๊ณผ immunopathological ๊ธฐ๋Šฅ์„ ์žฌํ˜„ ๋งˆ์šฐ์Šค์— ๋ฐ˜๋ณต์ ์œผ๋กœ ์ฃผ์ž… ์•„๋ฅด epidermolysis bullosa acquisita (EBA)<sup> 1</sup>.

Tags

Granulocyte-dependentAutoantibody-inducedSkin BlisteringAutoimmune PhenomenaSelf-toleranceAutoimmune ResponseWitebsky’s PostulatesDiagnosing Autoimmune DiseasePassive Transfer Of AutoantibodiesEpidermolysis Bullosa Acquisita (EBA)Experimental ModelsCollagen VIIBlistering PhenotypeHisto- And Immunopathological FeaturesHuman EBAWidespread DiseaseDose Of Administered Collagen VII-specific IgGGranulocyte Recruitment And ActivationInflammatory Pathway

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