/

/

病毒遗传背景明确的神经回路跟踪

JoVE 신문
신경과학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 신경과학

Viral Tracing of Genetically Defined Neural Circuitry

Please note that all translations are automatically generated. Click here for the English version.

病毒遗传背景明确的神经回路跟踪

DOI:

•

13:06 min

•

October 17, 2012

•

Kevin Beier, Constance Cepko

¹Department of Genetics,Harvard Medical School, ²Howard Hughes Medical Institute,Harvard Medical School

Chapters

00:05Title
01:16Passage and Concentration of Vesicular Stomatitis Virus (VSV)
04:23Stereotaxic Injection of Virus
08:19Tissue Harvest and Preparation for Microscopic Analysis
09:41Results: Behavior of rVSV(A/RG) Virus in Various Cell Types
12:22Conclusion

Summary

자동 번역

跟踪突触连接的神经元的方法。我们使用TVA特异性的上游电池，探测是否感兴趣的接收的细胞群体突触输入从基因定义的细胞类型。

Tags

Viral Tracing Genetically Defined Neural Circuitry Transsynaptic Viruses Pseudorabies Virus Rabies Virus Vesicular Stomatitis Virus Anterograde Direction Retrograde Direction Monosynaptic Retrograde Tracing Glycoprotein Gene Chimeric G TVA Receptor Inputs Onto A Defined Cell Type VSV As A Transsynaptic Tracer

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

由运动神经元调制生理，形态学和神经化学的表征

重复性小鼠坐骨神经挤压和再生的后续评估整装肌肉分析

扩展胚胎及成体神经干细胞<em>在子宫内</em电穿孔或病毒立体定向注射

禽流感胚胎听神经听觉神经纤维的选择性跟踪

建模星形细胞瘤发病机制<em>在体外</em>和<em>体内</em>使用星形胶质细胞或条件，遗传工程小鼠神经干细胞

新生大鼠于A气味偏侧学习模型解剖神经回路托换记忆的形成

有条件的遗传跨突触标记在胚胎小鼠脑

激光制导神经跟踪在脑外植体

视网膜神经节细胞定义子集的单细胞RNA-Seq

时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应

Read Article