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制备<em>果蝇</em>中央神经元<em在原位</em>膜片钳

JoVE 신문
신경과학

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JoVE 신문 신경과학

Preparation of Drosophila Central Neurons for in situ Patch Clamping

Please note that all translations are automatically generated. Click here for the English version.

制备<em>果蝇</em>中央神经元<em在原位</em>膜片钳

DOI:

•

08:27 min

•

October 15, 2012

•

Stefanie Ryglewski, Carsten Duch

¹School of Life Sciences,Arizona State University

Chapters

00:05Title
02:10Visualization of MN5
03:21Enzyme Pipette Preparation
05:08Cleaning of GFP-tagged MN5
07:02Results: In situ Patch Clamping of Drosophila Central Neurons
07:57Conclusion

Summary

자동 번역

原位补丁钳用于神经元的电生理特性的完整电路。在果蝇的遗传模型膜片钳是困难的，因为中枢神经系统，周围环绕着一个强大的护套。本文介绍的步骤，取出护套和清洁随后的膜片钳记录神经元。

Tags

Drosophila Central Neurons In Situ Patch Clamping Fruit Fly Drosophila Melanogaster Genetic Model Ion Channels Neuronal Excitability Voltage Gated Potassium Channel Shaker Genetic Tools Patch Clamp Recordings Drosophila CNS Glia And Collagen Sheath Patch Pipette Access Adult Drosophila CNS In Situ Patch Clamp Recordings Adult Brain Ventral Nerve Cord Stable Giga-seal Whole Cell Patch Clamp Recordings

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