JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:09Prepare Cannula and Syringe with PBS
- 02:13Prepare Mouse and Cannulate Extrahepatic Portal Vein or Common Bile Duct
- 04:40Resin Cast the Portal Vein or Intrahepatic Bile Duct
- 05:42Clear Liver for Visualization in situ
- 07:24Representative Liver Resin Casting
- 08:25Conclusion
Un método para visualizar y cuantificar la estructura 3-dimensional de la vena portal hepática del ratón o del conducto biliar intrahepático se describe. Esta técnica de colada de resina también se puede aplicar a otros sistemas de conductos o vascular y permite<em> In situ</em> Visualización o cuantificación de arquitectura intacta la comunicación de un sistema.
Tags
3-DimensionalResin CastingImagingMouse Portal VeinIntrahepatic Bile Duct SystemArchitectureVascular StructuresDuctal StructuresPhysiological FunctionFormationMaintenanceHighly Regulated ProcessAnalysis2-dimensional ExaminationSectionDye Injection StudiesComplete RepresentationQuantifiable RepresentationNovel TechniqueVisualizing StructuresQuantifying StructuresPlastic Resin CastsPermanent SnapshotConnected StructureCommunicating StructureBlood VesselDuct NetworkOrgan FunctionMorphological ChangesDisease States
