This content is Free Access.
Chapters
- 00:05Title
- 01:50Culturing the Macrophage Cell Line RAW 264.7
- 03:52Infection of RAW 264.7 Cells with MNV Inoculum
- 06:16Preparation of Low Melting Point Agarose Overlay
- 07:34Visualization of Plaques by Neutral Red Staining
- 08:49Results: Images of Cell Monolayers Before and After Infection and Plaque Formation
- 09:58Conclusion
Here we describe a method to quantify infectious particles of murine norovirus (MNV), which is the only norovirus that efficiently replicates in cell culture. The plaque assay takes advantage of MNV’s tropism for murine macrophages and can be adapted for use with biological or environmental samples containing MNV.
