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Chapters
- 00:05Title
- 02:00Helper-dependent Adenoviral Vector Production and Amplification
- 05:02Large Scale HDAd Production
- 07:13Vector Purification
- 11:19Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
- 13:08Evidence for Neo-islet Formation in Liver: Representative Results
- 16:14Conclusion
Nós descrevemos a formação de neo-hepática ilhota em STZ (estreptozotocina) induzida ratos diabéticos por transferência de genes de Neurogenin3 (Ngn3) e betacelulina (BTC), utilizando ajudante dependente do vector adenoviral (HDAD) e reversão da hiperglicemia. O nosso método tem vantagens do ajudante dependente de vectores adenovirais com a sua grande eficácia na transdução in vivo e a expressão do gene de longa duração.
Tags
Neo-Islet FormationLiverDiabetic MiceHelper-dependent Adenoviral Vector-Mediated Gene TransferType 1 DiabetesT Cell-mediated Autoimmune DestructionInsulin-producing CellsPancreasInsulin ReplacementIslet TransplantationDonor AvailabilityLong-term ImmunosuppressionInduced Islet NeogenesisGene TransferNeuogenin3 (Ngn3)Islet Lineage-defining Specific Transcription FactorBetacellulin (Btc)Islet Growth FactorAdenoviral Vectors (Ads)Gene Transfer VectorEarly Generation AdsSafety ProfileTransgene ExpressionChronic ToxicityViral Coding SequencesAd Cis ElementsVector Replication And PackagingProtocolHDAd-Ngn3HDAd-BtcSTZ-induced Diabetic Mice‘neo Islets’LiverHyperglycemia
