/

/

代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Please note that all translations are automatically generated. Click here for the English version.

代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体

DOI:

•

12:03 min

•

October 31, 2012

•

Andreia Gianotti Sommer, Sarah S. Rozelle, Spencer Sullivan, Jason A. Mills, Seon-Mi Park, Brenden W. Smith, Amulya M. Iyer, Deborah L. French, Darrell N. Kotton, Paul Gadue, George J. Murphy, Gustavo Mostoslavsky

¹Center for Regenerative Medicine (CReM),Boston University School of Medicine, ²Department of Hematology,Children’s Hospital of Philadelphia, ³Center for Cellular and Molecular Therapeutics,Children’s Hospital of Philadelphia

Chapters

00:05Title
01:50Isolation and Expansion of PBMCs
04:34Transduction of PBMCs with STEMCCA Lentivirus
06:11Plating Transduced Cells onto MEFs
08:41Picking and Expansion of iPSC Clones
09:26Results: Human Induced Pluripotent Stem Cells
11:26Conclusion

Summary

자동 번역

这里，我们表明一个简单而有效的协议，用于从外周血3-4毫升，使用一个单一的慢病毒重新编程矢量代人类iPSCs。现成的血细胞重新编程的承诺，加快利用IPSC的技术，使其接触到更广泛的研究团体。

Tags

Human Induced Pluripotent Stem Cells Peripheral Blood STEMCCA Lentiviral Vector Transcription Factors Oct4 Klf4 Sox2 CMyc Pluripotent State Embryonic Stem Cells Immune Rejection Disease Modeling Studies Pharmacological Compounds Screening Regenerative Therapies Transgene-free IPSCs Lung Diseases Skin Fibroblasts Reprogramming Peripheral Blood Cells

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生，强力霉素诱导慢病毒转导系统

由Stemgent人类TF慢病毒重新编程人类成纤维细胞生成诱导多能干细胞

慢病毒介导的击倒在体外红细胞

人类诱导多能干细胞的转染和Nucleofecting

选择和隔离殖民地的人类诱导多能干细胞重新编程成年成纤维细胞

人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体

诱导多能干细胞来源于小鼠的生成

人类多能干细胞的神经元在一个Multititre的板格式的快速和高效地生成

人类心肌细胞的生成：从馈线人类诱导多能干细胞的分化协议

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

Read Article