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Generation of Human induceret pluripotente stamceller fra perifert blod Brug af STEMCCA lentivirusvektor
 
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Generation of Human induceret pluripotente stamceller fra perifert blod Brug af STEMCCA lentivirusvektor

Article DOI: 10.3791/4327-v • 12:03 min • October 31st, 2012
October 31st, 2012 •
1, 1, 2, 3, 1, 1, 1, 3, 1, 3, 1, 1
1Center for Regenerative Medicine (CReM), Boston University School of Medicine, 2Department of Hematology, Children's Hospital of Philadelphia, 3Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia

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Her viser vi en enkel og effektiv protokol til generering af humane iPSCs 3-4 ml perifert blod under anvendelse af en enkelt lentiviral omprogrammering vektor. Omprogrammering af let tilgængelige blodlegemer lover at fremskynde udnyttelsen af ​​IPSC teknologi ved at gøre det tilgængeligt for et bredere forskningsmiljø.

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Stamcellebiologi induceret pluripotente stamceller (iPSCs) perifere mononukleare blodceller (PBMC'er) omprogrammering single udskæres lentiviral vektor STEMCCA
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