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1Biomedical Research Centre in Microbial Diseases,National Institute for Health Research, 2Respiratory Infection Group,Royal Liverpool and Broadgreen University Hospital Trust, 3Respiratory Infection Group,Liverpool School of Tropical Medicine, 4Institute of Infection and Global Health,University of Liverpool, 5Comprehensive Local Research Network,Royal Liverpool and Broadgreen University Hospital Trust, 6Department of Respiratory Research,University Hospital Aintree
Chapters
- 00:05Title
- 01:56Preparation for the Procedure
- 04:32Insertion and Positioning of the Bronchoscope
- 05:39Performing Bronchoalveolar Lavage
- 07:14Subject Recovery
- 08:13Isolation of Cells from Bronchoalveolar Lavage
- 10:33Results: Microscopic Observation of Cells Obtained by Bronchoalveolar Lavage
- 11:05Conclusion
Nous décrivons une technique de recherche pour la fibroscopie bronchique et lavage broncho-alvéolaire en utilisant aspiration basse pression. La technique est utilisée pour récolter des cellules immunitaires à partir des surfaces de broncho-pulmonaire. L'anesthésie locale et sédation consciente légère (midazolam) est utilisé. Sujets tolèrent les effets secondaires minimes procédure ainsi et d'expérience.
Tags
Bronchoalveolar LavageBALFiberoptic BronchoscopyResearch TechniqueManual SuctionNonadherent CellsLung Lining FluidMucosal SurfaceInnate ResponsesCellular ResponsesHumoral ResponsesLung MacrophageB-cellsT-cellsImmunoglobulinVolume InstillateCell DamageLocal AnestheticConscious SedationToleranceSide EffectsWritten Informed ConsentInclusion CriteriaExclusion Criteria
