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JoVE Journal Biology
The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
 

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Article DOI: 10.3791/4346-v • 10:41 min • January 13th, 2013
January 13th, 2013 •
1, 2, 1
1Chemical and Biological Engineering Department, State University of New York at Buffalo, 2Chemical Engineering Department, Massachusetts Institute of Technology

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요약
Automatic Translation

The heterologous biosynthesis of erythromycin A through E. coli includes the following experimental steps: 1) genetic transfer; 2) heterologous reconstitution; and 3) product analysis. Each step will be explained in the context of the motivation, potential, and challenges in producing therapeutic natural products using E. coli as a surrogate host.

Tags

Heterologous Natural Product Biosynthesis Complex Antibiotic Erythromycin A E. Coli Genetic Transfer Heterologous Reconstitution Product Analysis Biosynthetic Pathway Complexity Megasynthases PCR Isolation Operon Construction Multi-cystronic Plasmids Electro-transformation
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