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Isolating Primary Melanocyte-like Cells from the Mouse Heart
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Isolating Primary Melanocyte-like Cells from the Mouse Heart

Isolating Primary Melanocyte-like Cells from the Mouse Heart

DOI:
10.3791/4357-v

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07:38 min

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September 29, 2014

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, , ,
1Penn Cardiovascular Institute, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:31Preparation
  • 02:43Isolating Hearts from Neonatal Mouse Pups
  • 03:09Enzymatic Digestion
  • 04:25Plating Cardiac Melanocyte-like Cells
  • 05:24Isolating Cardiac Melanocyte-like Cells from Adult Mouse Heart
  • 06:25Results: Isolated Cardiac Melanocytes
  • 07:07Conclusion

Summary

자동 번역

자동 번역

In this protocol, we identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in mice. 

Tags

Primary Melanocyte-like CellsMouse HeartNovel PopulationCardiac MelanocytesAtrial Arrhythmia TriggersElectrical PropertiesBiological PropertiesIsolateProcedureNeonatal Murine CardiomyocytesPatch ClampBiochemical StudiesRefined ProcedureCutaneous MelanocytesPure PopulationCardiomyocytes

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