JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Chapters
- 00:00Title
- 01:18Introduction
- 03:16Preparation of Enzyme Solution
- 04:06Human Dental Pulp Isolation
- 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
- 06:28Outgrowth of Stem Cells from Tissue Explants
- 07:27Immunophenotyping
- 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
- 12:21Dental Pulp Stem Cells Culture and Differentiation Results
- 14:14Conclusion
विधि या तो का उपयोग करके और मानव दंत लुगदी स्टेम सेल (hDPSCs) के अलगाव के लक्षण वर्णन वर्णित<strong> लुगदी के enzymatic पृथक्करण (डीपीएससी प्रवर्तन निदेशालय)</strong> या<strong> गूदा ऊतक explants से स्टेम कोशिकाओं का सीधा परिणाम (डीपीएससी ओग). तो द्वारा पीछा</strong><em> इन विट्रो में</em> ओडॉन्टोब्लास्ट में hDPSCs के दोनों प्रकार के तुलनात्मक भेदभाव.
Tags
IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk
