Journal
/
면역학 및 감염병학
/
Isolation of Lymphocytes from Mouse Genital Tract Mucosa
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Isolation of Lymphocytes from Mouse Genital Tract Mucosa

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

DOI:
10.3791/4391-v

•

04:46 min

•

September 03, 2012

•

,
1Department of Pathology and Laboratory Medicine, David Geffen School of Medicine,University of California, Los Angeles , 2California NanoSystems

Chapters

  • 00:05Title
  • 01:12Removal of the Genital Tract
  • 02:15Digesting Genital Tract Tissue
  • 03:08Separating Genital Tract Cells Using Percoll Gradients
  • 03:59Representative Results
  • 04:22Conclusion

Summary

자동 번역

자동 번역

An efficient way to isolate lymphocytes from mouse genital tract is described. This method takes advantage of enzyme digestion and Percoll gradient separation to allow efficient isolation. This technique is also adaptable to for use in other species

Tags

IsolationLymphocytesMouse Genital Tract MucosaMucosal SurfacesGastrointestinalUrogenitalRespiratory TractsPathogensVirusesBacteriaMucosaeImmunityPeyers PatchesReproductive Mucosal SitesSexually Transmitted Diseases (STD)Bacterial InfectionsViral InfectionsCenters For Disease Control And PreventionHealth Care SystemReproductive Mucosal ImmunityIsolating LymphocytesMurine Female Genital Tracts

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Transurethral Induction of Mouse Urinary Tract Infection

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Activation of Murine Lymphocytes

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

Read Article