This content is Free Access.
Chapters
- 00:05Title
- 01:06Preparation of Native Collagen Fibrils
- 01:45Preparation of Fibrous Long Spacing Collagen
- 02:35Preparation of Segmental Long Spacing Collagen
- 03:19Atomic Force Microscopy Characterization of Collagen Fibrils
- 05:10Results: AFM Verification of Collagen Fibril Type
- 07:15Conclusion
Enkle og reproducerbare procedurer er beskrevet til fremstilling af tre strukturelt forskellige collagen samlinger fra en fælles kommercielt tilgængelig type I collagen monomer. Native type, kan fibrøst lange mellemrum eller segmenterede lang afstand collagen konstrueres ved at variere de betingelser, som de 300 nm lang og 1,4 nm diameter monomer byggesten er blotlagt.
Tags
In Vitro SynthesisNative CollagenFibrous Long Spacing CollagenSegmental Long Spacing CollagenExtracellular MatrixStructural ScaffoldingMechanical StrengthCollagen FibrilsBanding PeriodicityType I Collagen MonomersHierarchical AssemblyLength ScalesMicronsCommercially Available Collagen MonomerProtocolsMixtures Of StructuresUnbanded FibrilsImagingAtomic Force Microscope
