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从长期来看，高解析度激光共聚焦时间流逝成像<em>拟南芥子叶</em>表皮萌发过程中

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Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

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从长期来看，高解析度激光共聚焦时间流逝成像<em>拟南芥子叶</em>表皮萌发过程中

DOI:

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12:01 min

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December 31, 2012

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Kylee M. Peterson, Keiko U. Torii^2,3

¹Department of Biology,University of Washington, ²Howard Hughes Medical Institute,University of Washington, ³PRESTO,Japan Science and Technology Agency

Chapters

00:05Title
01:00Seed Sterilization
01:57Preparation of Chamber Slide
03:11Seed Dissection
04:47Mounting Cotyledons in the Chamber Slide
05:40Time Lapse Imaging
09:29Results: Changes in Localization of POLAR Protein during Proliferation Stage of Stomatal-lineage Cells in the Cotyledon Epidermis
11:40Conclusion

Summary

자동 번역

我们描述了一个协议，用玻片和媒体的固定几天的发展，记录植物子叶共聚焦成像的表皮气孔分化。萤光标记的蛋白质，可以动态跟踪的表达和亚细胞定位，细胞分裂和细胞分化过程中增进了解其可能的作用。

Tags

Confocal Time Lapse Imaging Arabidopsis Cotyledon Cellular Behavior In Vivo Dynamics Plant Development Long-term Live Imaging Cell Division Differentiation Events Arabidopsis Epidermis Signaling Cell Fate Stomata Asymmetric Division Cell Differentiation Steps

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