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Chapters
- 00:05Title
- 01:27Assembly of the Microfluidic Culture Chamber (MCP)
- 02:43Preparation of Neuronal Culture
- 03:33Induction of Neuronal Axons in Assembled MCP
- 04:48A Compartmentalized Co-culture System: Adding Cultured Astrocytes
- 06:15Results: Axon-induced GLT1 Promoter Activation in Astrocytes
- 09:07Conclusion
This study describes the procedures of setting up a novel neuronal axon and (astro)glia co-culture platform. In this co-culture system, manipulation of direct interaction between a single axon (and single glial cell) becomes feasible, allowing mechanistic analysis of the mutual neuron to glial signaling.
Tags
Imaging AnalysisNeuronGlia InteractionMicrofluidic Culture PlatformMCP-based Neuronal Axon And Glia Co-culture SystemSignaling PathwaysCentral Nervous System (CNS)Neuron And Glia Mixed CulturesNeuronal CompartmentsAxonDendriteSomaCulture SystemSoluble FactorsDirect Membrane Contact SignalsSingle AxonGlial Cell
