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Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:54Preparation of Lymphocytes
- 02:43Preparation of Alloantigen
- 03:46Cell Preparation for Injections
- 05:01Pre-measurement, Injection, and Post-measurement
- 07:18Results: Trans-vivo Delayed Type Hypersensitivity Assay
- 10:56Conclusion
We describe a valuable diagnostic assay that could potentially be used to decide the withdrawal of immunosuppression after transplant without elevated risk of graft rejection. The assay uses the principles of Delayed Type Hypersensitivity and provides accurate assessment of both donor specific effector and regulatory immune responses mounted by recipients.
Tags
Trans-vivoDelayed-type Hypersensitivity AssayAntigen-specific RegulationT Cell-dependent Immune ResponseForeign AntigenSensitization PhaseChallenge PhaseAnergyTraditional DTH AssayMicrobial InfectionsTransplant PatientsDirect DTHDiagnostic TechniqueHuman-to-mouseTransfer DTH AssayPeripheral Blood Mononuclear Cells (PBMCs)Specific AntigensNaïve MouseSwelling MeasurementAntigen PresentationMacrophagesDCsInflammatory Cascade
