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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

DOI:
10.3791/50034-v

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10:04 min

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October 29, 2012

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, ,
1Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

Chapters

  • 00:05Title
  • 01:46Acute Brain Slice Preparation and Patch Clamp Recording
  • 03:47Local Application of Drugs to Neurons in Slices
  • 06:07Controlling the Drug-filled Micropipette with a Piezoelectric Translator
  • 07:22Results: Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
  • 09:33Conclusion

Summary

자동 번역

자동 번역

In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

Tags

Nicotinic Acetylcholine ReceptorNAChRNicotine AddictionElectrophysiologyWhole-cell Patch ClampBrain SlicesLocal Drug ApplicationNicotineAcetylcholineMouseNeuron

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