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Chapters
- 00:05Title
- 01:49Stage-specific Injection of Morpholinos (MO)
- 03:10Selecting Injected Embryos for Analysis
- 04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
- 05:56Visualization and Analysis of KV Fluid Flow
- 06:38Representative Kupffer’s Vesicle Fluid Flow Results
- 07:45Conclusion
Cilia-gegenereerde vloeistofstroom in Blaasjes Kupffer's (KV) regelt links-rechts patroonvorming van de zebravis embryo. We beschrijven een techniek voor het moduleren genfunctie specifiek in KV cellen. Verder tonen we hoe fluorescerende kralen leveren in KV om vloeistofstroming visualiseren.
