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Chapters
- 00:05Title
- 01:49Stage-specific Injection of Morpholinos (MO)
- 03:10Selecting Injected Embryos for Analysis
- 04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
- 05:56Visualization and Analysis of KV Fluid Flow
- 06:38Representative Kupffer’s Vesicle Fluid Flow Results
- 07:45Conclusion
Cilia-generated Fluidströmung in Kupffer-Vesikel (KV) steuert links-rechts Strukturierung des Zebrafisch-Embryos. Hier beschreiben wir ein Verfahren zur Modulation Genfunktion spezifisch in Zellen KV. Darüber hinaus zeigen wir, wie fluoreszierende Kügelchen in KV liefern Strömung zu visualisieren.
