/

의학

/

利用双光子显微镜在慕尼黑Wistar大鼠量化肾小球通透性荧光大分子

JoVE 신문
의학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 의학

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Please note that all translations are automatically generated. Click here for the English version.

利用双光子显微镜在慕尼黑Wistar大鼠量化肾小球通透性荧光大分子

DOI:

10.3791/50052-v

•

11:13 min

•

April 17, 2013

•

Ruben M. Sandoval, Bruce A. Molitoris

¹Medicine/Nephrology,Indiana University School of Medicine

Chapters

00:05Title
01:50Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
04:10Acquiring Images to Quantify Renal Permeability of Albumin
06:02Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
08:21Glomerular Permeability Results
10:34Conclusion

Summary

자동 번역

利用高分辨率intavital 2 - 光子显微镜的技术直接可视化和量化表面肾小球过滤gloemrular。此方法允许在正常和疾病状态的大分子渗透特性的直接测定。

Tags

Keywords: 2-photon Microscopy Glomerular Permeability Fluorescent Macromolecules Munich Wistar Rats Kidney Diseases Urinary Loss Serum Albumin Podocytes Vascular Endothelial Cells Basement Membrane Glomerular Filtration Barrier Proximal Tubule Cells Micropuncture Dextran Polymers Bowman’s Space Albumin Reabsorption Tubular Transcytosis Proteinuric Renal Diseases

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

瘦素受体<sup>分贝</sup> 2型糖尿病小鼠模型：胰岛分离和活细胞双光子成像完好胰岛

应用先进的背皮窗室对转基因荧光小鼠肿瘤相关血管的活体显微镜观察

一种使用加速度计量化上肢表现在日常生活的方法

体内Menière 病耳和正常听觉的磁共振成像对人颅神经的形态学分析

小鼠肾小球滤过率的透皮测定

高灵敏度测量与氟素等西洋盐-聚糖小鼠的球状渗透性 70

小鼠窦房和房室结的全卡口免疫荧光染色、共聚焦成像和 3D 重建

小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养

分析长期心电图记录以检测小鼠心律失常

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Read Article