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Chapters
- 00:05Title
- 01:50Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
- 04:10Acquiring Images to Quantify Renal Permeability of Albumin
- 06:02Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
- 08:21Glomerular Permeability Results
- 10:34Conclusion
Una técnica de la utilización de alta resolución intavital 2 fotones microscopía de visualizar directamente y cuantificar la filtración gloemrular en los glomérulos superficie. Este método permite la determinación directa de las características de permeabilidad de macromoléculas tanto en estados normales y enfermos.
Tags
Keywords:
2-photon MicroscopyGlomerular PermeabilityFluorescent MacromoleculesMunich Wistar RatsKidney DiseasesUrinary LossSerum AlbuminPodocytesVascular Endothelial CellsBasement MembraneGlomerular Filtration BarrierProximal Tubule CellsMicropunctureDextran PolymersBowman’s SpaceAlbumin ReabsorptionTubular TranscytosisProteinuric Renal Diseases
