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Chapters
- 00:05Title
- 01:10Preparation of Chromatin
- 03:58Chromatin Immunoprecipitation (ChIP)
- 08:38Analysis
- 12:04Results: DNA Enrichment from Primary Lymphocytes using ChIP
- 13:52Conclusion
We describe a robust method for chromatin immunoprecipitation using primary T cells. The method is founded on standard approaches, but uses a specific set of conditions and reagents that improve efficiency for limited a quantities of cells. Importantly, a detailed description of the data analysis phase is presented.
