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Chapters
- 00:05Title
- 02:32Dissection of Cortical Tissue
- 04:51Plating Mixed Cortical Cells
- 06:38Obtaining an Enriched Astrocyte Culture
- 09:11Results: Morphology and Purity of Astrocyte Cultures
- 10:53Conclusion
Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.
