Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Single Cell Measurements of Vacuolar Rupture…
JoVE Journal
Immunology and Infection

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Immunology and Infection
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
 

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Article DOI: 10.3791/50116-v • 10:39 min • June 12th, 2013
June 12th, 2013 •
*1, *1, 2, 3, 3, 1, 1
1Dynamique des Interactions Hôte Pathogène, Institut Pasteur, Paris, France, 2Imagopole, Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée, Institut Pasteur, Paris, France
* These authors contributed equally

챕터

요약
Automatic Translation

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

Tags

Keywords: Single Cell Vacuolar Rupture Intracellular Pathogens Shigella Flexneri Mycobacterium Tuberculosis CCF4 FRET Reporter Phagosomal Membrane Disruption Real-time Imaging High-throughput Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter