JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:06Inclusion Body Preparation and Nickel Matrix Binding
- 03:55On-column Oxidative Crosslinking
- 04:50TFA Elution and Quantitation
- 05:52CNBr Digestion
- 07:54Reversed-phase HPLC Purification of Disulfide-crosslinked Peptide Complexes
- 09:06TrpLE fusions: Transmembrane Peptide Complexes Expressed in Bacteria
- 11:19Conclusion
Biophysical and biochemical studies of interactions among membrane-embedded protein domains face many technical challenges, the first of which is obtaining appropriate study material. This article describes a protocol for producing and purifying disulfide-stabilized transmembrane peptide complexes that are suitable for structural analysis by solution nuclear magnetic resonance (NMR) and other analytical applications.
